The first book printed in the Americas was a catechism in Spanish and Nahuatl, published in 1539. The title page pictured here is taken from a later work, the bi-lingual catechism of Pedro de Gante, the Doctrina Christiana en lengua mexicana of 1553.
Pedro de Gante (ca. 1479–1572), Doctrina Christiana en lengua mexicana, title page, Mexico, 1553; source: University of Texas at Austin, Benson Latin American Collection, http://instructional1.calstatela.edu/bevans/Art454L-01-MapsDocsEtc/WebPage-Info.00003.html.